Natl Sci Open
Volume 2, Number 3, 2023
|Number of page(s)
|Life Sciences and Medicine
|30 March 2023
Single-cell protein-DNA interactomics and multiomics tools for deciphering genome regulation
State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China
2 Peking University-Tsinghua University-National Institute of Biological Science Joint Graduate Program, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China
3 Institute of Molecular Medicine, College of Future Technology, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China
Revised: 22 December 2022
Accepted: 9 January 2023
The emergence of single-cell genomic and transcriptomic sequencing accelerates the development of single-cell epigenomic technologies, providing an unprecedented opportunity for decoding cell fate decisions largely encoded in the epigenome. Recent advances in single-cell multimodality epigenomic technologies facilitate directly interrogating the regulatory relationship between multi-layer molecular information in the same cell. In this review, we discuss recent progress in development of single-cell multimodality epigenomic technologies and applications in elucidating cellular diversifications in development and diseases, with a focus on protein-DNA interactomics and regulatory links between epigenome and transcriptome. Further, we provide perspective on the future direction of single-cell multiomics tool development as well as challenges facing ahead.
Key words: single-cell protein-DNA interaction / single-cell multiomics / single-cell epigenomics / epigenome / genome regulation
© The Author(s) 2023. Published by Science Press and EDP Sciences
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