Natl Sci Open
Volume 2, Number 5, 2023
Special Topic: Gene Editing towards Translation
|Number of page(s)||14|
|Section||Life Sciences and Medicine|
|Published online||19 April 2023|
Anthocyanin-assisted Agrobacterium infiltration for the rapid evaluation of genome editing efficiencies across multiple plant species
State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China
2 College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100101, China
3 College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
Revised: 26 December 2022
Accepted: 26 December 2022
CRISPR-based genome editing technologies continue to drive major advances in life sciences. A major challenge for realizing widespread use of genome editing in plants and agriculture is establishing methods that enable the rapid, comprehensive, and precise evaluation of editing technologies using transient methods. Here we report a new and rapid genome editing evaluation method using Agrobacterium infiltration techniques to enable broad-spectrum, simplistic, and precise assessments of genome editing efficiencies. We employed an anthocyanin marker to facilitate visual screenings of genome-edited cells for use in adult strawberry fruits as well as tomato fruits, cotton leaves, and sugar beet leaves. Using this method, we demonstrate the ability to quickly measure genome editing efficiencies mediated by SpCas9, LbCas12a, A3A-PBE, ABE8e, and PPE. This new method will allow researchers to rapidly and easily evaluate genome editing tools across a broad spectrum of plant species, further expediting the development of genome-edited agricultural crops.
Key words: genome editing / Agrobacterium infiltration / anthocyanin / transient transformation / geminivirus replicon / strawberry / tomato
© The Author(s) 2023. Published by Science Press and EDP Sciences.
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