ZY001 inhibited the expression of immunosuppressive molecules in Raw 264.7 cells. Mouse Raw 264.7 macrophages were treated with E7046 (10 μM) or ZY001 (0.1, 1, 10 μM) in the existence of PGE₂ (40 nM) for 24 h. The mRNA expression of Il4 (A), Mrc1 (B), Chil3 (C), Trem2 (D) and Ptgs2 (E) were determined by Q-PCR. Gapdh was used as a reference gene. Data were shown as the mean ± SEM. One-way ANOVA followed by Tukey’s post hoc tests were performed; *P < 0.05; **P < 0.01; ***P < 0.001; and ****P < 0.0001.